Mycoplasma Broth Base w/ CV (PPLO Broth Base w/ CV)

PPLO Media were described by Morton, Smith and Leberman. It was used in a study of the growth requirements of Mycoplasma, along with the identification and cultivation of this organism. Pivotal information regarding Mycoplasma has been documented by Sabin. Hayflick et al have reported the information regarding the cultivation of Mycoplasma. For the cultivation of Mycoplasma, the medium ingredients and all the supplements should be free of any toxic substances even in small amounts. Many Mycoplasma require serum for their good growth and also presence of antibiotic is necessary to prevent the growth of contaminating organisms.