Kligler Iron Agar in Pakistan
| Typical Formula* |
gm/litre |
| `Lab-Lemco’ powder |
3.0 |
| Yeast extract |
3.0 |
| Peptone |
20.0 |
| Sodium chloride |
5.0 |
| Lactose |
10.0 |
| Glucose |
1.0 |
| Ferric citrate |
0.3 |
| Sodium thiosulphate |
0.3 |
| Phenol red |
0.05 |
| Agar |
12.0 |
Description
Kligler Iron Agar, Oxoid Preparation:
Suspend 55g in 1 litre of distilled water. Bring to the boil to dissolve completely. Mix well and distribute into containers. Sterilise by autoclaving at 121°C for 15 minutes. Allow to set as slopes with 1 inch butts.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.
Precautions
It is essential that Kligler Iron Agar is examined and reported at 18-24 hours. Early or late readings will give false results.
KIA will grow both oxidative and fermentative organisms. Confusion will result if care is not taken to distinguish between the two groups.
Always use a straight wire to inoculate the butt, to avoid splitting the agar with a loop.
Pure cultures are essential to avoid misinterpretation.
Do not use screw-capped tubes or bottles for KIA medium. It is essential that air is freely available to growth on the slant.
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