Blood Agar Base in Pakistan

Description

Usages:
For the growth of pathogenic and non-pathogenic microorganisms.

Principle:
Polypeptone provide carbon and nitrogen sources, vitamins and growth factors; Neisseria starch can promote the growth characteristics and enhanced hemolytic streptococci; sodium chloride to maintain osmotic balance; agar as culture medium coagulant.

Formulation (per liter):
Peptone: 10g
Beef extract powder :10g
Sodium chloride: 5g
Agar :15g
Final pH 7.4 ± 0.2

How to use:
1. Suspend 40g of the product, adding 1 L of distilled or deionized water, ,heated to boiling stirring until completely dissolved, dispensing into flask, 121 autoclaved 15min.
2.After the medium is cooled to about 50 , sterile procedures, basal medium was added per 100mL defibrinated sheep or rabbit blood 5-10mL, shake pour plate; also dispensing sterile tubes set into the slope. Spare.
3. take the bacteria to be tested fresh pure cultures inoculated crossed or coated on the plate, set the temperature of the incubator training requirements were cultured for a predetermined time.
4. Observe the results.